20:21 Oct 31, 2022 |
|
English to Serbian translations [PRO] Medical - Medical: Pharmaceuticals / exon skipping | |||||||
---|---|---|---|---|---|---|---|
|
|
Summary of answers provided | ||||
---|---|---|---|---|
4 | stepen/nivo/mera promene genske ekspresije |
|
Discussion entries: 3 | |
---|---|
stepen/nivo/mera promene genske ekspresije Explanation: Izostavljena je bitna rečenica koja prethodi citiranom delu izvornog teksta, jer se pomenuta RT-PCR tehnika koristi upravo za merenje genske ekspresije. "FIG. 19 provides a bar graph of the percentage of exon 45 skipping in healthy human myoblasts by PMO #1 and PPMO #1 at various concentrations 96 hours after treatment, as measured by RT-PCR." Ima prilično referenci, pa se unapred izvinjavam, ako sajt to negde usput skrati/blokira zbog ograničenja. "Since the invention of real-time PCR (qPCR), thousands of high-impact studies have been conducted and published using qPCR technique (Heid et al. 1996; Higuchi et al. 1993; VanGuilder, Vrana, and Freeman 2008). ... IV. FOLD CHANGE IN QPCR In every well, the qPCR experiment measures the expression intensity of a certain gene from a sample under specific biological conditions. ... Comparing the normalized expression (∆Ct) of the two conditions it is possible to calculate the fold change of the expression of the miRNA (−∆∆Ct). The fold change is the expression ratio: if the fold change is positive it means that the gene is upregulated; if the fold change is negative it means it is downregulated (Livak and Schmittgen 2001)." https://gene-quantification.de/integromics-qpcr-statistics-w... "Gene Expression Level ... Quantification of expression levels To quantify the differences in gene expression levels, an expression ratio can be computed. This ratio can be either a simple ratio (e.g., disease/healthy), or a log-transformed ratio [e.g., log (disease/healthy)]. The advantage of the simple ratio is their intuitive understanding, although they treat up- and down-regulated transcripts differently (e.g., upregulation by a factor of 2: 2/1 = 2; downregulation by a factor of 2: 1/2 = 0.5)." https://www.sciencedirect.com/topics/biochemistry-genetics-a... "Pri izboru adekvatnog referentnog gena, ispitivali smo stabilnost ekspresije gena za GAPDH (gliceraldehid-3-fosfat), ACTB (β-aktin), 18S (18S ribozomalna RNK), HPRT (hipoksantin-guanin-fosforibozil-transferaza) i TBP (TATA boks vezujući protein). Stabilnost ispitivanih referentnih gena je evaluirana korišćenjem ΔCt metoda, kao i upotrebom dva softvera NormFinder (129) i BestKeeper (130). [...] Rezultati su predstavljeni kao N-fold promene genske ekspresije normalizovane u odnosu na ekspresiju GAPDH." https://nardus.mpn.gov.rs/bitstream/id/77091/Disertacija_116... "Promena u ekspresiji (fold change) u tumorskom tkivu u odnosu na neizmenjeno tkivo od istog bolesnika je klasifikovana po sledećem kriterijumu: fold change >1 kao „povećana”, fold change |
| |
Login to enter a peer comment (or grade) |
Login or register (free and only takes a few minutes) to participate in this question.
You will also have access to many other tools and opportunities designed for those who have language-related jobs (or are passionate about them). Participation is free and the site has a strict confidentiality policy.