Glossary entry

English term or phrase:

cup spinning sequenator

Serbian translation:

(Edmanov/proteinski) sekvenator/sekvencer

Added to glossary by Svetomir Mijovic
Jul 18, 2022 11:15
1 yr ago
13 viewers *
English term

cup spinning sequenator

English to Serbian Medical Medical: Pharmaceuticals tumor antibodies
...the polypeptide will be purified ...by use of a cup spinning sequenator,

Proposed translations

10 hrs
English term (edited): spinning-cup sequenator/sequencer
Selected

(Edmanov/proteinski) sekvenator/sekvencer

"Edman later developed the spinning-cup sequencer in the 1970s. The machine used liquid reagents, which meant that a lot of protein was lost during washing. As a result, it required micrograms worth of starting material, an amount that was usually prohibitive for anything other than an abundant protein. The method could sequence only a few amino acids at once. “By the time you got to 30 amino acids, you were done for,” says Gottesfeld. “You couldn't resolve the next amino acid from the noise of all the previous amino acids.”

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5663895

"Edmanov metod degradacije se može automatizovati u uređajima koji se nazivaju sekvenatori gde se automatski sprovodi 40 do 50 ciklusa odvajanja i identifikacije aminokiselina. Duži polipeptidni lanci se ne mogu sekvencionirati odjednom kao takvi nego selektivno razlažu na manje segmente pomoću specifičnih enzima i tako dobijeni segmenti se podvrgavaju Edmanovoj degradaciji."

http://polj.uns.ac.rs/sites/default/files/udzbenici/HEMIJA.p...

"Dvije glavne izravne metode sekvenciranja proteina su masovna spektrometrija i Edmanova degradacija pomoću a proteinski sekvenator (sekvencer)"

https://wikihrhr.top/wiki/Protein_sequencing

"Za identifikaciju HPLC-om, snimi se hromatogram svih standardnih aminokiselina sa kojima se porede aminokiseline dobijene Edmanovom degradacijom. Pri Edmanovoj degradaciji C-terminalni kraj se obično veže za nerastvoran nosač. Instrument koji se koristi je Edmanov sekvenator (engl. Edman sequenator)."

https://sh.wikipedia.org/wiki/Primarna_struktura_proteina

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