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Can temperature of buffer used when diluting a protein ...www.researchgate.net › post-

A 10mM His buffer formulated at pH 6.2 (at 25*C) reads 6.4-6.5 when removed from a 2-8*C cooler. If you were to use this cold buffer to dilute a mAb which is ...

10mM His buffer, 100mM arginine hydrochloride, protein concentration 20mg/ml, pH=6.2;
10mM His buffer, 100mM proline, protein concentration 20mg/ml, pH=6.2;
10mM His buffer, 100mM NaCl, protein concentration 20mg/ml, pH=6.2;
10mM His buffer, 4.2% mannitol, protein concentration 20mg/ml, pH=6.2;
10mM His buffer, 50mM arginine hydrochloride, 2% sucrose, protein concentration 20mg/ml; pH=6.2;