碱基

06:35 Feb 26, 2022
This question was closed without grading. Reason: Answer found elsewhere

Chinese to English translations [PRO]
Medical - Medical: Health Care / Cancer of a woman
Chinese term or phrase: 碱基
碱基质量q30占比

DNA测序深度

Base? Nucleobase?

Thanks!
Gleyse
France
Local time: 12:25


Summary of answers provided
4base
Yawen Xing
4Q30
Randy Wong


Discussion entries: 1





  

Answers


1 hr   confidence: Answerer confidence 4/5Answerer confidence 4/5
base


Explanation:
It is fine to use "base" as the translation of 碱基 in a sequencing report, some bioinfo companies like Illumina also use "base" in its official website.

For Q30, it is an indicator for the probability of error occurred in 碱基识别 (base calling). Higher Q-score means higher accuracy. More explanation could be found at:

http://blog.sina.com.cn/s/blog_6e856ac10102vouh.html
https://zhuanlan.zhihu.com/p/364968563
https://www.illumina.com/science/technology/next-generation-...

Example sentence(s):
  • Q20值是指的测序过程碱基识别(Base Calling)过程中,对所识别的碱基给出的错误概率。
Yawen Xing
China
Local time: 18:25
Native speaker of: Chinese
PRO pts in category: 1
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17 hrs   confidence: Answerer confidence 4/5Answerer confidence 4/5
Q30


Explanation:
Q30
表示一个碱基的质量值,也表示该碱基错误率百分比。Q30指碱基质量值为30,错误率为0.1%,正确率为99.99%。同理Q40表示错误率为0.01%,正确率为99.999%。

二代测序中,每测一个碱基会给出一个相应的质量值,这个质量值是衡量测序准确度的。碱基的质量值13,错误率为5%,20的错误率为1%,30的错误率为0.1%。行业中Q20与Q30则表示质量值≧20或30的碱基所占百分比。例如一共测了1G的数据量,其中有0.9G的碱基质量值大于或等于20,那么Q20则为90%。

首先,碱基质量值是衡量测序质量的重要指标,质量值(Q)越高代表碱基被测错的概率(P)越小,其计算公式为Q=-10lgP。



Example sentence(s):
  • https://www.jianshu.com/p/d67cb5ec68ba
  • https://zhuanlan.zhihu.com/p/364968563
Randy Wong
China
Local time: 18:25
Works in field
Native speaker of: Native in ChineseChinese
PRO pts in category: 16
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