Jan 3, 2021 19:14
3 yrs ago
22 viewers *
English term

To gate

English to French Science Biology (-tech,-chem,micro-) Instrument (Gene Transfection) / Fluorescence imagery
J'ai un doute sur le terme "to gate" dans cette phrase : "This allows Users to visualize the cells they are gating on and set distinct thresholds between GFP+ and GFP- cells". C'est flou.

Merci :)
Proposed translations (French)
5 Filtrer
4 -1 trier ou stratégie de gating
Votes to reclassify question as PRO/non-PRO:

Non-PRO (1): Lionel-N

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Discussion

Nathalie Beaudelot Jan 4, 2021:
Who is gating in the sentence? Who is they? the Users? Why a capital letter at users?

Proposed translations

-1
32 mins

trier ou stratégie de gating

Universal and Efficient Electroporation Protocol for Genetic ...
www.jove.com › ...
18 févr. 2020 — Détermination de l'efficacité de la transfection ... Numérisation des cellules activées fluorescentes (FACS) ... Analyser les cellules à l'aide d'une machine FACS à l'aide d'une stratégie de gating appropriée (voir la figure 3; ...

Anisotropie de fluorescence - Thèses
www.theses.fr › ...
PDF
15 déc. 2011 — GFP constitue une sonde fluorescente ayant un rendement quantique ... (protocole de transfection, Annexe III.1), des expériences de transfert d'énergie (FRET), etc… ... photocathode soit sur la « gating mesh » (Fig.1.10).
de V Devauges · ‎2011 · ‎Autres articles
Note from asker:
Merci !
Peer comment(s):

disagree Lionel-N : Absolument pas! C’est pas du sorting mais du Gatting//Manu, tous les FACS ne sont pas des cell sorter; tous font du gatting (filtrage d'un marqueur), mais pas du sorting (triage de cellules préalablement marquées).
3 hrs
Trier = filtrer sorting est synonyme de gating
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3 hrs

Filtrer

Vocabulaire élémentaire en FACS

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Note added at 3 heures (2021-01-03 22:52:37 GMT)
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Gatting c’est filtrer, à savoir utiliser une « gate «  pour evidencier les cellules a une certaine longueur d’onde selon ce que l’on veut justement evidencier; le sorting (cell sorting) c’est le triage qui permet de récupérer les cellules triées ; ça n’a aucun rapport! Dans le premier cas on quantifie ce qui est présent et on jette la préparation, dans le cell sorting on purifie et récupère les cellules pour successif traitement. Ici aussi c’est du basic de chez basic!
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