RFC-null recipient cells

English to German Medical Genealogy SELECTION SYSTEM FOR EUKARYOTIC CELL CULTURE BASED ON A MEMBRANE-BOUND FOLATE RECEPTOR GENE

There are several disadvantages for the RFC selection system: a) One must use ***RFC-null recipient cells*** in which the endogenous RFC locus has been knocked out or inactivated by targeted knockout or loss of function mutations. b) RFC has an extremely poor transport affinity for folic acid and thus this oxidized folate cannot be used for selection. c) As opposed to the current folate-receptor based system that is a unidirectional folate uptake system and which will be explained in detail below, RFC is a bi-directional folate transporter that exhibits equally potent import and export of folates.