Glossary entry (derived from question below)
English term or phrase:
Dde I restriction digestion
Lithuanian translation:
virškinimas apribojant Dde I
Added to glossary by
Kristina Radziulyte
Jul 31, 2012 07:46
11 yrs ago
English term
restriction digestion
English to Lithuanian
Science
Biology (-tech,-chem,micro-)
We used Dde I enzyme to determine the heterogeneity of campylobacter strains isolated in broilers because Dde I restriction digestion provides the best discrimination for veterinary isolates
Proposed translations
(Lithuanian)
2 | virškinimas apribojant Dde I | Gintautas Kaminskas |
Change log
Aug 2, 2012 09:47: Gintautas Kaminskas Created KOG entry
Aug 2, 2012 09:57: Kristina Radziulyte changed "Edited KOG entry" from "<a href="/profile/6089">Gintautas Kaminskas's</a> old entry - "restriction digestion"" to ""virškinimas apribojant Dde I""
Proposed translations
21 mins
English term (edited):
Dde I restriction digestion
Selected
virškinimas apribojant Dde I
Abstract
Protein 4.1 is a major component of the junctional complex at the red cell skeleton. Genomic studies have recently evidenced that the encoding gene (EL1 locus) is present in a single copy per haploid genome. Several RFLPs have already been characterized within intron sequences. Here, we describe the first RFLP found within the coding sequence. This polymorphism (C or T at position 2723, in exon 21) does not affect the amino acid sequence (Thr-->Thr). It can be detected by either Dde I restriction digestion of an appropriate PCR product, or simply by SSCP These findings should facilitate analysis of families with 4.1 deficiencies causing hereditary elliptocytosis.
tHIS ABstract from http://www.ncbi.nlm.nih.gov/pubmed/9554757 shows that the words "Dde I restriction digestion of an appropriate PCR product" belong together in the phrase just mentioned.
Protein 4.1 is a major component of the junctional complex at the red cell skeleton. Genomic studies have recently evidenced that the encoding gene (EL1 locus) is present in a single copy per haploid genome. Several RFLPs have already been characterized within intron sequences. Here, we describe the first RFLP found within the coding sequence. This polymorphism (C or T at position 2723, in exon 21) does not affect the amino acid sequence (Thr-->Thr). It can be detected by either Dde I restriction digestion of an appropriate PCR product, or simply by SSCP These findings should facilitate analysis of families with 4.1 deficiencies causing hereditary elliptocytosis.
tHIS ABstract from http://www.ncbi.nlm.nih.gov/pubmed/9554757 shows that the words "Dde I restriction digestion of an appropriate PCR product" belong together in the phrase just mentioned.
4 KudoZ points awarded for this answer.
Comment: "Ačiū."
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