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22:27 Mar 9, 2016 |
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Spanish to English translations [PRO] Medical - Biology (-tech,-chem,micro-) / microbiología | |||||||
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| Selected response from: Muriel Vasconcellos United States Local time: 05:51 | ||||||
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Discussion entries: 4 | |
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Bacteria: spread plate 10 ml of the 1:10 dilution Explanation: Volume to Volume - Wellesley College academics.wellesley.edu/Biology/Concepts/Html/volumetovolume.html 25 Aug 2004 - For example, to make a 1:10 dilution of a 1M NaCl solution, you would ... For example: if you needed 10 mL of the 1:10 dilution, then you would ... -------------------------------------------------- Note added at 21 mins (2016-03-09 22:48:20 GMT) -------------------------------------------------- Genetic Transformation Systems in Fungi https://books.google.co.uk/books?isbn=3319105035 Marco A. van den Berg, Karunakaran Maruthachalam - 2014 - Science a10 μg of DNA in X μL (plus DNA) or X μL of water (minus DNA; as a control) ... glass tubes (about 15 mL in each) and dilute with equal volume of 1×STC. ... (d) 100 μL+900 μL of 1 ×STC; spread 100 μL of this 1:10 diluted sample (S1) on ... -------------------------------------------------- Note added at 21 mins (2016-03-09 22:48:56 GMT) -------------------------------------------------- or just Bacteria: spread 10 ml of the 1:10 dilution |
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Bacteria: inoculation from 10 ml of the 1:10 dilution Explanation: I think inoculation is perfectly fine here, and I imagine it's done from 10 ml rather than with, since using the whole 10 ml would be quite a lot for two agar plates. Bacteria may be introduced to the media (inoculated) by various means. Usually the bacteria e.g. from a drop in a heat-sterilised loop are spread on the surface of (ready set) agar. http://www.biotopics.co.uk/microbes/tech1.html Un microorganismo se puede sembrar en un medio líquido o en la superficie de un medio sólido de agar. https://es.wikipedia.org/wiki/Cultivo_(microbiología) Once the growth medium in the petri dish is inoculated with the desired bacteria, the plates are incubated at the best temperature for the growing of the selected bacteria (for example, usually at 37 degrees Celsius for cultures from humans or animals, or lower for environmental cultures). https://en.wikipedia.org/wiki/Microbiological_culture |
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Bacteria: inocuation of 10 mL of/from the 1:10 dilution Explanation: IMO, you were correct to begin with. -------------------------------------------------- Note added at 4 hrs (2016-03-10 03:06:26 GMT) -------------------------------------------------- Here is an excerpt about the spread plate technique: http://www.microbelibrary.org/component/resource/laboratory-... One method of distributing bacteria evenly over the surface of an agar plate medium is commonly referred to as the spread plate method. Classically a small volume of a bacterial suspension is spread evenly over the agar surface using a sterile bent glass rod as the spreading device. The goal in evenly distributing the bacterial suspension is typically to permit the growth of colonies that can subsequently be enumerated (see Serial Dilution Protocols) and/or sampled following incubation. Each plate is spread with a single inoculum of the bacterial suspension. An alternative approach to spreading a single inoculum volume with a smooth device is to apply a smaller volume and tip the plate, allowing gravity to distribute the inoculum in a band or track (track method) or to allow the inoculum to dry in place (drop method). With this alternative approach, several sample dilutions can be distributed on a single agar plate. |
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